Fluorescence of pyoverdine synthesized by Pseudomonas under the effect of iron oxide nanoparticles

The fluorescent emission of the thermolyzed Pseudomonas aeruginosa suspensions was measured in order to get a quantitative indicator upon the pyoverdine synthesis – as molecular basis of iron acquisition system of the bacterial cells. The influence of iron ions on the dynamics of pyoverdine was studied by supplying nano-sized iron oxide suspensions in the bacteria culture medium. The stimulatory effect of both iron oxide levels tested within this experiment (10 microl/l and 100 microl/l) was emphasized during the first 14 hours of incubation. The diminution of the pyoverdine synthesis was suggested for 20 and 22 incubation hours, but only in the case of the magnetite suspension highest level (of 100 microl/l). Potential applications to the experimental study of environmental pollution of the Pseudomonas species – known as having various ecological niches with magnetic nanoparticles could be developed based on the results provided in this study.